Difference between revisions of "Part:BBa K590016"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K590016 short</partinfo> | <partinfo>BBa_K590016 short</partinfo> | ||
− | + | This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit]. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part consists of mamI gene from ''Magnetospirillum magneticum'' strain AMB-1, sfGFP in the backbone of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590011 pGA1C3]. It was made by Gibson Cloning Method. | ||
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+ | It was previously reported that the loss of mamI causes no membrane formation, and it functions as a localization protein onto chains. | ||
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+ | This part was transformed into ''E.coli'' and membrane localization (particularly the poles) was observed: | ||
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+ | [[Image:SfGFP-I-1C3- uwigem2011.jpg|600px|center]] | ||
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Revision as of 06:04, 19 September 2011
sfGFP_mamI_pGA1C3
This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].
Usage and Biology
This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. It was made by Gibson Cloning Method.
It was previously reported that the loss of mamI causes no membrane formation, and it functions as a localization protein onto chains.
This part was transformed into E.coli and membrane localization (particularly the poles) was observed:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 80