Difference between revisions of "Part:BBa K608101:Design"
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Yuu Hirose ''et al'' ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein."<br/>''Proc Natl Acad Sci U S A''. 105: 9528–9533. | Yuu Hirose ''et al'' ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein."<br/>''Proc Natl Acad Sci U S A''. 105: 9528–9533. | ||
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+ | SUPPLEMENTAL INFORMATION | ||
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+ | Tabor, J. J. ''et al.''(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. , doi:10.1016/j.jmb.2010.10.038 |
Latest revision as of 21:10, 20 September 2011
CcaR, green light response regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 380
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We deleted an EcoRI restriction site in CcaR, in order to make it compatible to the iGEM standart.
Source
It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803. The following primers were used: XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG and ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG
References
Yuu Hirose et al ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein."
Proc Natl Acad Sci U S A. 105: 9528–9533.
SUPPLEMENTAL INFORMATION from Tabor, J. J. et al.(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. , doi:10.1016/j.jmb.2010.10.038