Difference between revisions of "Part:BBa K546000:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K546000 short</partinfo>
 
<partinfo>BBa_K546000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
+
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: [https://parts.igem.org/Part:BBa_K546003|K546003].
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 18:29, 16 September 2011

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


Design Notes

The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: [1].

Source

The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.

References