Difference between revisions of "Part:BBa K118022:Experience"
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Both substrates produce a fluorescent product when cleaved. Our plates below show the results of placing cell lysate and cell debris on an MUG plate and an MUC plate. Present on both plates are: | Both substrates produce a fluorescent product when cleaved. Our plates below show the results of placing cell lysate and cell debris on an MUG plate and an MUC plate. Present on both plates are: | ||
| − | * Left side of plate: JM109 expressing ''bglX'', <partinfo>BBa_K523014</partinfo> | + | * Left side of plate: lysate/debris from JM109 expressing ''bglX'', <partinfo>BBa_K523014</partinfo> |
| − | * Right side of plate: JM109 expressing ''cex'', <partinfo>BBa_K523016</partinfo> | + | * Right side of plate: lysate/debris from JM109 expressing ''cex'', <partinfo>BBa_K523016</partinfo> |
| − | * Bottom of plate: JM109 cells | + | * Bottom of plate: lysate/debris from JM109 cells |
<center> | <center> | ||
Revision as of 20:22, 7 October 2011
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how you used this part and how it worked out.
Applications of BBa_K118022
Characterization
Edinburgh 2011 conducted two assays, comparing the activity of this part (but under the control of the lac promoter, giving BBa_K523016) to a β-glucosidase (E. coli bglX, also under the control of the lac promoter) on two different substrates:
- 4-methylumbelliferyl β- D- glucuronide (MUG, left photo). This substrate is a cellobiose analog.
- 4-methylumbelliferyl β- D- cellobioside (MUC, right photo). This substrate is larger and is more like a cellulose analog.
Both substrates produce a fluorescent product when cleaved. Our plates below show the results of placing cell lysate and cell debris on an MUG plate and an MUC plate. Present on both plates are:
- Left side of plate: lysate/debris from JM109 expressing bglX, BBa_K523014
- Right side of plate: lysate/debris from JM109 expressing cex, BBa_K523016
- Bottom of plate: lysate/debris from JM109 cells
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| MUG assay. bglX on left, cex on right. | MUC assay. bglX on left, cex on right. |
As can be seen, cex is much better at degrading MUC.
User Reviews
UNIQ6391bdcdf80bf926-partinfo-00000003-QINU UNIQ6391bdcdf80bf926-partinfo-00000004-QINU