Difference between revisions of "Part:BBa K678000"
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DMKP-P6 is a newly identified constitutive ''Aspergillus nidulans'' promoter of medium strength. | DMKP-P6 is a newly identified constitutive ''Aspergillus nidulans'' promoter of medium strength. | ||
| − | + | [[Image:Img0003-51.jpg|frame|none|Figure 1: As can be seen on the plate we have two positive controls the strong PgpdA 0.5kb and PgpdA 1.0kb promoters and a negative control that does not posses the lacZ gene. By comparing the intensities of the blue color we can see that DMKP-P6 is most likely a promoter of medium strength. To further investigate this we performed a β-galactosidase assay.]] | |
https://static.igem.org/mediawiki/igem.org/0/07/Img0003-51.jpg | https://static.igem.org/mediawiki/igem.org/0/07/Img0003-51.jpg | ||
Revision as of 11:29, 13 September 2011
DMKP-P6, Aspergillus nidulans promoter
DMKP-P6 is a newly identified constitutive Aspergillus nidulans promoter of medium strength.
File:Img0003-51.jpg
Figure 1: As can be seen on the plate we have two positive controls the strong PgpdA 0.5kb and PgpdA 1.0kb promoters and a negative control that does not posses the lacZ gene. By comparing the intensities of the blue color we can see that DMKP-P6 is most likely a promoter of medium strength. To further investigate this we performed a β-galactosidase assay.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 23
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 23
Illegal NheI site found at 473 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 23
Illegal XhoI site found at 122
Illegal XhoI site found at 132 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 23
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 23
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 212
Illegal BsaI.rc site found at 499