Difference between revisions of "Part:BBa K515100"
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<partinfo>BBa_K515100 short</partinfo>
 
<partinfo>BBa_K515100 short</partinfo>
 
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<p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) <a href="https://parts.igem.org/Part:BBa_K515000">BBa_K515000</a>,  catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) <a href="https://parts.igem.org/Part:BBa_K515001">BBa_K515001</a> . There are several different pathways that produce indole-3-acetic acid. IaaM and IaaH originate from <i>P.savastanoi</i> and have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]</p>
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<p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) <a href="https://parts.igem.org/Part:BBa_K515000">BBa_K515000</a>,  catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) <a href="https://parts.igem.org/Part:BBa_K515001">BBa_K515001</a> . There are several different pathways that produce indole-3-acetic acid (add image). IaaM and IaaH originate from <i>P.savastanoi</i> and have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant. [1]</p>  
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<b>Compatability</b>
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<p>Chassis: This device has been tested in <i>E. coli</i> strain DH5α as a construct assembled with the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone vector. </p>  
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<h2>References</h2>
 
<h2>References</h2>
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[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. <i>Journal of Bacteriology</i>, 171(2), pp.1002-1009.</p>
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<p>[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. <i>Journal of Bacteriology</i>, 171(2), pp.1002-1009.</p>

Revision as of 17:51, 13 September 2011

IAA biosynthetic genes under control of the Pveg2 promoter

The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) BBa_K515000, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) BBa_K515001 . There are several different pathways that produce indole-3-acetic acid (add image). IaaM and IaaH originate from P.savastanoi and have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant. [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
    Illegal BamHI site found at 1492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 254
    Illegal NgoMIV site found at 2835
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 171(2), pp.1002-1009.