Difference between revisions of "Part:BBa K515100"
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| − | <p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. We are expressing genes of the IAM pathway that originate from <i>P.savastanoi</i> in <i>E. coli</i>. IaaM and IaaH have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]</p> | + | <p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) <a href="https://parts.igem.org/Part:BBa_K515000">BBa_K515000</a>, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) <a href="https://parts.igem.org/Part:BBa_K515001">BBa_K515001</a> . There are several different pathways that produce indole-3-acetic acid. We are expressing genes of the IAM pathway that originate from <i>P.savastanoi</i> in <i>E. coli</i>. IaaM and IaaH have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]</p> |
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| − | + | <b>Compatability</b> | |
| + | <p>Chassis: This device has been tested in <i>E. coli</i> strain | ||
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Revision as of 13:52, 12 September 2011
IAA biosynthetic genes under control of the Pveg2 promoter
The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) BBa_K515000, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) BBa_K515001 . There are several different pathways that produce indole-3-acetic acid. We are expressing genes of the IAM pathway that originate from P.savastanoi in E. coli. IaaM and IaaH have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]
CompatabilityChassis: This device has been tested in E. coli strain Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 547
Illegal BamHI site found at 1492 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 254
Illegal NgoMIV site found at 2835 - 1000COMPATIBLE WITH RFC[1000]