Difference between revisions of "Part:BBa K515100"
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<partinfo>BBa_K515100 short</partinfo>
 
<partinfo>BBa_K515100 short</partinfo>
  
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<p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. [1] We are expressing genes of the IAM pathway that originate from Psuedomonas savastanoi in E. coli. IaaM and IaaH have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. </p>  
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<p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. We are expressing genes of the IAM pathway that originate from <i>P.savastanoi</i> in <i>E. coli</i>. IaaM and IaaH have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]</p>  
  
  
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<partinfo>BBa_K515100 parameters</partinfo>
 
<partinfo>BBa_K515100 parameters</partinfo>
 
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<h2>References</h2>
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<p>[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. <i>Journal of Bacteriology</i>, 171(2), pp.1002-1009.</p>

Revision as of 13:26, 12 September 2011

IAA biosynthetic genes under control of the Pveg2 promoter

The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. We are expressing genes of the IAM pathway that originate from P.savastanoi in E. coli. IaaM and IaaH have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
    Illegal BamHI site found at 1492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 254
    Illegal NgoMIV site found at 2835
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 171(2), pp.1002-1009.