Difference between revisions of "Part:BBa K592008:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter. | + | The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed, slowing bacterial growth. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter. |
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===Source=== | ===Source=== |
Revision as of 22:02, 11 September 2011
T5-lac Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed, slowing bacterial growth. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
Source
Phage T5