Difference between revisions of "Part:BBa K515102:Experience"

Revision as of 15:30, 10 September 2011

Characterisation

This part (BBa K515102) has been characterised qualitatively and quantitatively for chemotactic response towards L (-) malic acid. The tests were performed across a range of different attractant concentrations to observe optimal functioning of the part. Qualitative tests were performed using semi solid agar assays, quantitative analysis was performed using high - throughput capillary assay with cell count for each capillary evaluated using FACS. Behavioral analysis of E. coli expressing this part was done using Wide - field microscopy, and manual tracking was used to evaluate tactic response. Negative control used was E. coli 5α strain with a selectable marker for ampicillin and kanamycin. As positive control chemotactic response of E. coli 5α strain to attractant L (-) serine was tested, as serine is compatible with endogenous chemotaxis system of E. coli 5α strain.

Qualitative assay

User Reviews

UNIQ0f7038376f07f26a-partinfo-00000000-QINU UNIQ0f7038376f07f26a-partinfo-00000001-QINU

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 __NOTOC__
 <p><b>Characterisation</b></p>
-<p>This part (BBa K515102) has been characterised qualitatively and quantitatively for chemotactic response towards L (-) malic acid. The tests were performed across a range of different attractant concentrations to observe optimal functioning of the part. Qualitative tests were performed using semi solid agar assays, quantitative analysis was performed using high - throughput capillary assay with cell count for each capillary evaluated using FACS. Behavioral analysis of E. coli expressing this part was done using Wide - field microscopy, and manual tracking was used to evaluate tactic response.</p>
+<p>This part (BBa K515102) has been characterised qualitatively and quantitatively for chemotactic response towards L (-) malic acid. The tests were performed across a range of different attractant concentrations to observe optimal functioning of the part. Qualitative tests were performed using semi solid agar assays, quantitative analysis was performed using high - throughput capillary assay with cell count for each capillary evaluated using FACS. Behavioral analysis of E. coli expressing this part was done using Wide - field microscopy, and manual tracking was used to evaluate tactic response. Negative control used was <i>E. coli 5α strain</i> with a selectable marker for ampicillin and kanamycin. As positive control chemotactic response of <i>E. coli 5α strain</i> to attractant L (-) serine was tested, as serine is compatible with endogenous chemotaxis system of <i>E. coli 5α strain</i>. </p>
+<p><b>Qualitative assay</b></p>
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K515102 StartReviews</partinfo>