Difference between revisions of "Part:BBa K515000"

Revision as of 12:34, 9 September 2011

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, Psuedomonas savastanoi which produces and secretes indole-3-acetic acid (auxin).

The sequence has been codon optimised for E. coli and B. subtilis and is under control of the pVEG promoter with an RBS also suitable for E. coli and B. subtilis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 450
Illegal BamHI site found at 1395
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 157
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K515000 short</partinfo>
-IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. The sequence for this is from <i>Psuedomonas savastanoi</i> which uses a two-enzyme pathway to produce indole 3 acetic acid (auxin).
+IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, <i>Psuedomonas savastanoi</i> which produces and secretes indole-3-acetic acid (auxin).
 The sequence has been codon optimised for <i>E. coli</i> and <i>B. subtilis</i> and is under control of the pVEG promoter with an RBS also suitable for <i>E. coli</i> and <i>B. subtilis</i>.