Difference between revisions of "Part:BBa K538004:Experience"

Revision as of 19:07, 21 September 2011

Figure 1. Percentage of surviving cells after 1, 2 and 3 freeze/thaw cycles for E. coli that contains a CspC generator (blue) or an empty control vector (black).

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Applications of BBa_K538004

This brick's effect on E. coli 's cold resistance was characterised by its developers, team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]. Expressing it via BBa_K538204, they looked into 1) whether or not expression of CspC influences growth rate at suboptimal temperatures, and 2) if E. coli comprising this brick experiences enhanced freeze/thaw cycle survival.

CspC is a cold-induced chaperone, so it's conceivably beneficial to growth rate at low temperatures. However, there are no indications of this in literature, so there were no strong a priori expectations about this brick's influence on growth rate. It has been reported CspC strongly increases E. coli 's ability to survive freeze/thaw cycles.[www.springerlink.com/index/QR2Q5722J0T78653.pdf]

Expression of this brick wasn't found to have a significant effect on E. coli 's growthrate at suboptimal temperatures, which is as we expected, but not what we'd hoped for. We did, however, manage to reproduce the cold resistant phenotype described by Uh et al. in a freeze/thaw cycle experiment whose results are displayed in figure 1.

Uh et al. Rescue of a Cold-Sensitive Mutant at Low Temperatures by Cold Shock Proteins from Polaribacter irgensii KOPRI 22228 J. Microbiol. 48, 798-802 (2010)

User Reviews

UNIQbb7860cf621defde-partinfo-00000000-QINU UNIQbb7860cf621defde-partinfo-00000001-QINU

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 __NOTOC__
+[[Image:Freeze_thaw_graph.png|right|frame|'''Figure 1.''' Percentage of surviving cells after 1, 2 and 3 freeze/thaw cycles for ''E. coli'' that contains a ''CspC'' generator (blue) or an empty control vector (black).]]
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
 ===Applications of BBa_K538004===
+This brick's effect on ''E. coli'' 's cold resistance was characterised by its developers, team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]. Expressing it via [[Part:BBa_K538204|BBa_K538204]], they looked into '''1)''' whether or not expression of ''CspC'' influences growth rate at suboptimal temperatures, and '''2)''' if ''E. coli'' comprising this brick experiences enhanced freeze/thaw cycle survival.
+''CspC'' is a cold-induced chaperone, so it's conceivably beneficial to growth rate at low temperatures. However, there are no indications of this in literature, so there were no strong ''a priori'' expectations about this brick's influence on growth rate. It ''has'' been reported ''CspC'' strongly increases ''E. coli'' 's ability to survive freeze/thaw cycles.[www.springerlink.com/index/QR2Q5722J0T78653.pdf]
+Expression of this brick wasn't found to have a significant effect on ''E. coli'' 's growthrate at suboptimal temperatures, which is as we expected, but not what we'd hoped for. We '''did''', however, manage to reproduce the cold resistant phenotype described by Uh ''et al.'' in a freeze/thaw cycle experiment whose results are displayed in figure 1.
+# '''Uh ''et al.''''' Rescue of a Cold-Sensitive Mutant at Low Temperatures by Cold Shock Proteins from ''Polaribacter irgensii'' KOPRI 22228 ''J. Microbiol. 48, 798-802'' (2010)
 ===User Reviews===