Difference between revisions of "Part:BBa K676001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The Mu phage Gyrase Binding sites serve as a high affinity binding site for the DNA gyrase enzymes. The site will be cleaved preferentially by the GyrA and resulting in an introduction of linking number difference into the DNA by the topoisomerase. | ||
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| + | Apparently some bacteriophages exploited the GBS to allow them to replicate successfully in their host. For example, Mu – bacteriophage has a strong gyrase site or GBS in the central region of the 37.2kbp Mu phage genome. | ||
| + | From Pato(1990) it was found out that the wild type phage can carry out replication effectively compare to the mutant which lacks the GBS after the heat induction of the lysogens . | ||
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Revision as of 11:12, 21 September 2011
Gyrase Binding Site from Mu Bacteriophage
The strong gyrase site clones from Mu bacteriophage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000COMPATIBLE WITH RFC[1000]