Difference between revisions of "Part:BBa J100031:Design"
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https://parts.igem.org/Part:BBa_J100028. We used this website to design and attach sticky ends to our oligo sequences as shown. | https://parts.igem.org/Part:BBa_J100028. We used this website to design and attach sticky ends to our oligo sequences as shown. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 14:39, 1 September 2011
Constitutive promoter C on Gene 1 of T7, transcribes RNA Pol.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
https://parts.igem.org/Part:BBa_J100028. We used this website to design and attach sticky ends to our oligo sequences as shown.
Source
We found the sequence of the oligo sequence from the paper as well.
McConnell DJ. The DNA sequence at the T7 C promoter. Biological Labs, Harvard University. 29 November 1978. Nucleic Acids Research.