Difference between revisions of "Part:BBa K549001"

 
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<partinfo>BBa_K549001 short</partinfo>
 
<partinfo>BBa_K549001 short</partinfo>
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E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.
 
E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.
  
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E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.
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===Usage and Biology===
 
===Usage and Biology===
  
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This BioBrick can be used to detect Ni(II)-Ions in water.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:21, 20 September 2011

Ni(II) dependent rcnA-promoter and GFP

E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.

E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.

Usage and Biology

This BioBrick can be used to detect Ni(II)-Ions in water.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863