Difference between revisions of "Production/Competent Cells"
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<tr><th>Batch<th>Strain<th>Maker<th>Date Started<th>Temp, RPM<th>Incubation (hr)<th>Culture Volume<th>Culture OD (600nm) | <tr><th>Batch<th>Strain<th>Maker<th>Date Started<th>Temp, RPM<th>Incubation (hr)<th>Culture Volume<th>Culture OD (600nm) | ||
<th>Competent OD (600nm)<th>Cell Count (AVG)<th>Efficiency<th>250ul Aliquots<th>500ul Aliquots<th>1ml Aliquots</tr> | <th>Competent OD (600nm)<th>Cell Count (AVG)<th>Efficiency<th>250ul Aliquots<th>500ul Aliquots<th>1ml Aliquots</tr> | ||
| − | <tr><td>001<td>NEB 10b<td>VS+KN< | + | <tr><td>001<td>NEB 10b<td>VS+KN<td>8/17/11<td>21C, 250rpm<td>16<td>500ml<td>0.4 |
<td>1.35<td>67,88,112 (89)<td>1.335*10^8<td>20<td>20<td>10 | <td>1.35<td>67,88,112 (89)<td>1.335*10^8<td>20<td>20<td>10 | ||
</table> | </table> | ||
Revision as of 15:09, 19 August 2011
Batch Summary
Use this page to keep track of competent cell production. See the competent cell protocol for more information.
| Batch | Strain | Maker | Date Started | Temp, RPM | Incubation (hr) | Culture Volume | Culture OD (600nm) | Competent OD (600nm) | Cell Count (AVG) | Efficiency | 250ul Aliquots | 500ul Aliquots | 1ml Aliquots |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 001 | NEB 10b | VS+KN | 8/17/11 | 21C, 250rpm | 16 | 500ml | 0.4 | 1.35 | 67,88,112 (89) | 1.335*10^8 | 20 | 20 | 10 |
Batch Notes
001
This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch. A resounding success!