Difference between revisions of "Production/Competent Cells"
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==Batch Notes== | ==Batch Notes== | ||
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| − | This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch. A resounding success | + | This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch. A resounding success! |
Revision as of 15:08, 19 August 2011
Batch Summary
Use this page to keep track of competent cell production. See the competent cell protocol for more information.
| Batch | Strain | Maker | Date Started | Temp, RPM | Incubation (hr) | Culture Volume | Culture OD (600nm) | Competent OD (600nm) | Cell Count (AVG) | Efficiency | 250ul Aliquots | 500ul Aliquots | 1ml Aliquots |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 001 | NEB 10b | VS+KN | 8/17/11 | 21C, 250rpm | 16 | 500ml | 0.4 | 1.35 | 67,88,112 (89) | 1.335*10^8 | 20 | 20 | 10 |
Batch Notes
001
This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch. A resounding success!