Difference between revisions of "Production/Competent Cells"
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Revision as of 15:01, 19 August 2011
Batch Summary
Use this page to keep track of competent cell production. See the competent cell protocol for more information.
| Batch | Strain | Date Started | Temp, RPM | Incubation (hr) | Culture Volume | Culture OD (600nm) | Competent OD (600nm) | Cell Count w/ (AVG) | Efficiency | 250ul Aliquots | 500ul Aliquots | 1ml Aliquots |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 001 | NEB 10b | 8/17/11 | 21C, 250rpm | 16 | 500ml | 0.4 | 1.35 | 67,88,112 (89) | 1.335*10^8 | 20 | 20 | 10 |