Difference between revisions of "Part:BBa K584016"

 
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<partinfo>BBa_K584016 short</partinfo>
 
<partinfo>BBa_K584016 short</partinfo>
  
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More information will be added soon.
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We performed a '''PCR''' to get the '''CeaB''' part of the '''K131009 biobrick'''. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product.  This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lac-Lux hybrid promotor to get  this biobrick, ‘Lac-Lux hybrid promotor’ + Ribolock + ceaB + B0015.
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The primer sequence we used in the PCR reaction is -
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ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC
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ceaB-RV: AAAACTGCAGCGGCCGCTACTAGTCTCTAGTATTACTTACCCCGATGAATATCAATAT
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:55, 19 September 2011

Lac-Lux hybrid promoter + Ribolock-CeaB + Terminator

We performed a PCR to get the CeaB part of the K131009 biobrick. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product. This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lac-Lux hybrid promotor to get this biobrick, ‘Lac-Lux hybrid promotor’ + Ribolock + ceaB + B0015.

The primer sequence we used in the PCR reaction is -

ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC

ceaB-RV: AAAACTGCAGCGGCCGCTACTAGTCTCTAGTATTACTTACCCCGATGAATATCAATAT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]