Difference between revisions of "Part:BBa K091104:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 21: | Line 21: | ||
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K091104 AddReview number</partinfo> | <partinfo>BBa_K091104 AddReview number</partinfo> | ||
| − | <I>SarahV</I> | + | <I>SarahV, KULeuven iGEM team 2011</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | ||
Revision as of 20:01, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091104
User Reviews
UNIQ584f668c7fa5e22b-partinfo-00000000-QINU
|
No review score entered. SarahV, KULeuven iGEM team 2011 |
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp. |
UNIQ584f668c7fa5e22b-partinfo-00000002-QINU