Difference between revisions of "Part:BBa K523011"
Line 6: Line 6:
 
BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
 
BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
  
−
The signal peptide of the endoglucanase (amino acids 1 - 31) has been removed, since this part is intended for fusion to INP or other display proteins.  
+
The signal peptide of the endoglucanase (amino acids 1 - 31) has been removed, since this part is intended for fusion to INP or other display proteins. Nevertheless a start codon is included so this part can also be used as a control.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:19, 8 August 2011

Endoglucanase for BioSandwich

This is a modified version of BBa_K118023 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

The signal peptide of the endoglucanase (amino acids 1 - 31) has been removed, since this part is intended for fusion to INP or other display proteins. Nevertheless a start codon is included so this part can also be used as a control.

Usage and Biology

This is an endoglucanase from Cellulomonas fimi. It degrades cellulose chains by cutting them in the middle, producing smaller chains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 141
    Illegal XhoI site found at 503
    Illegal XhoI site found at 752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 187