Difference between revisions of "Part:BBa K523007"
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''E. coli'' periplasmic alpha-amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening. | ''E. coli'' periplasmic alpha-amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening. | ||
| − | This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see <partinfo>BBa_K523003</partinfo>) mean there's not even a stop codon provided by the BioBrick suffix. | + | This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see <partinfo>BBa_K523003</partinfo>) mean there's not even a stop codon provided by the BioBrick suffix, since it's now in a different frame. |
This part might therefore not be of any use to anyone, even ourselves... | This part might therefore not be of any use to anyone, even ourselves... | ||
Revision as of 12:35, 29 July 2011
Plac + LacZ + malS (dubious version)
E. coli periplasmic alpha-amylase gene malS under control of lac promoter. LacZ is also present for blue/white screening.
This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see BBa_K523003) mean there's not even a stop codon provided by the BioBrick suffix, since it's now in a different frame.
This part might therefore not be of any use to anyone, even ourselves...
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1830
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 625
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 966
Illegal AgeI site found at 2131 - 1000COMPATIBLE WITH RFC[1000]