Difference between revisions of "Part:BBa K523007"

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''E. coli'' periplasmic alpha-amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening.
 
''E. coli'' periplasmic alpha-amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening.
  
This contains an artificial RBS, and no stop codon...
+
This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see <partinfo>BBa_K523003</partinfo> mean there's not even a stop codon provided by the BioBrick suffix.
 +
 
 +
This part might therefore not be of any use to anyone, even ourselves...
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:32, 29 July 2011

Plac + LacZ + malS (dubious version)

E. coli periplasmic alpha-amylase gene malS under control of lac promoter. LacZ is also present for blue/white screening.

This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see BBa_K523003 mean there's not even a stop codon provided by the BioBrick suffix.

This part might therefore not be of any use to anyone, even ourselves...

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 966
    Illegal AgeI site found at 2131
  • 1000
    COMPATIBLE WITH RFC[1000]