Difference between revisions of "Part:BBa K606027:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K606027 short</partinfo>
 
<partinfo>BBa_K606027 short</partinfo>
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===Design Notes===
 
===Design Notes===
 +
 
Switch from pSB1AK3 plasmid to pSB1C3
 
Switch from pSB1AK3 plasmid to pSB1C3
 
 
  
 
===Source===
 
===Source===
  
YFP:tetR comes from Andrew Wright, Dave Lane, pFX234 plasmid.
+
Andrew Wright, Dave Lane, François-Xavier Barre, pFX234 plasmid.
  
 
===References===
 
===References===
 +
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997<br>
 +
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004

Latest revision as of 21:47, 21 October 2011

pVeg-SpoVG-YFP-tetR-Term for B. subtilis and E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Switch from pSB1AK3 plasmid to pSB1C3

Source

Andrew Wright, Dave Lane, François-Xavier Barre, pFX234 plasmid.

References

Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004