Difference between revisions of "Part:BBa K265008:Experience"

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===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K265008 AddReview number</partinfo>
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<partinfo>BBa_K415151</partinfo>
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<I>Edinburgh 2011</I>
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<I>Allan Crossman (Edinburgh 2011)</I>
 
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Edinburgh can confirm that the sequence of the part itself is as expected. The part comes in RFC12 format and therefore has an unusual prefix and suffix. We found an extra C base in the suffix at the end of the NotI site:
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Edinburgh successfully fused this part to Enhanced Yellow Fluorescent Protein to produce <partinfo>BBa_K523013</partinfo>. Since the cells fluoresced, the INP did not prevent correct folding.
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<pre>
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Expected: GCTAGC GCGGCCG CTGCAG
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Actual:  GCTAGC GCGGCCGC CTGCAG
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</pre>
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Revision as of 12:22, 8 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K265008

User Reviews

UNIQ1a2dd5af0e5ef4ca-partinfo-00000000-QINU

BBa_K415151 Allan Crossman (Edinburgh 2011)

Edinburgh successfully fused this part to Enhanced Yellow Fluorescent Protein to produce BBa_K523013. Since the cells fluoresced, the INP did not prevent correct folding.

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UNIQ1a2dd5af0e5ef4ca-partinfo-00000003-QINU