Difference between revisions of "Part:BBa I13522:Experience"

Revision as of 00:07, 28 October 2010

For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.
Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.

The characterization was performed by METU-TURKEY IGEM 2010 Team.

Applications of BBa_I13522

User Reviews

UNIQ3456e91dcddcdd85-partinfo-00000001-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ3456e91dcddcdd85-partinfo-00000003-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+<br> For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.
-how you used this part and how it worked out.
+<br> Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.
+<html> <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 608px; height: 316px;" alt="w7" src="https://static.igem.org/mediawiki/2010/6/66/C2.GIF"></a></div> </html>
+The characterization was performed by METU-TURKEY IGEM 2010 Team.
 ===Applications of BBa_I13522===