Difference between revisions of "Part:BBa K523002"
(→‎Usage and Biology)
Line 10: Line 10:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
The product protein is believed to be periplasmic. A beta-glucosidase cleaves Β1-4 bonds, i.e. those found in the disaccharide cellobiose.
+
The product protein is believed to be periplasmic. A beta-glucosidase cleaves Β(1→4) bonds, i.e. those found in the disaccharide cellobiose.
  
  

Revision as of 15:36, 19 July 2011

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 after site-specific mutagenesis has been carried out.

Usage and Biology

The product protein is believed to be periplasmic. A beta-glucosidase cleaves Β(1→4) bonds, i.e. those found in the disaccharide cellobiose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
    Illegal AgeI site found at 1880
    Illegal AgeI site found at 2069
  • 1000
    COMPATIBLE WITH RFC[1000]