Difference between revisions of "Part:BBa K523002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K523002 short</partinfo> | <partinfo>BBa_K523002 short</partinfo> | ||
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The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The product protein is believed to be periplasmic. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K523002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K523002 SequenceAndFeatures</partinfo> |
Revision as of 15:16, 19 July 2011
RBS + bglX (E. coli perisplasmic β-glucosidase)
This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
Usage and Biology
The product protein is believed to be periplasmic.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1658
Illegal AgeI site found at 1880
Illegal AgeI site found at 2069 - 1000COMPATIBLE WITH RFC[1000]