Difference between revisions of "Part:BBa K523001:Design"
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===Source=== | ===Source=== | ||
− | The | + | The source ''E. coli'' genome sequence can be seen using GenBank U00096.2 |
===References=== | ===References=== |
Latest revision as of 14:45, 19 July 2011
RBS + malS (E. coli periplasmic α-amylase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1233
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 369
Illegal AgeI site found at 1534 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
The part was created from a lab E. coli strain using primers:
- Forward: ACT AGATCT gaa atc gca gca ata agg act c
- adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of BBa_K523000 in a normal BioBrick vector.
- Reverse: ACT ACTAGT A TTA tta ctg ttg ccc tgc cca g
- adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon.
Source
The source E. coli genome sequence can be seen using GenBank U00096.2