Difference between revisions of "Part:BBa R0062"

Revision as of 13:53, 16 June 2012

Promoter (luxR & HSL regulated -- lux pR)

Promoter activated by LuxR in concert with HSL

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

This Plux promoter "pointing to the right" is the same sequence, but inverted, as part BBa_K199052.

Usage and Biology

Pretty good off in the absence of LuxR/HSL. [jb, 5/24/04]
The team from Davidson College and Missouri Western State University discovered that this part promotes "backwards transcription" when LuxR protein is present and AHL-3OC6 is absent. You can read [http://www.ibc7.org/article/journal_v.php?sid=265 the paper that documents this unexpected "backwards promoter activity"] in their open access paper.

Sequence and Features