Difference between revisions of "Part:BBa M36706"
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| − | IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. | + | IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. Therefore when pyruvate is present in high concentrations, the aceBAK promoter should be highly inhibited and downstream gene expression should be low. When pyruvate levels are low, the aceBAK promoter should be less inhibited and the downstream genes should be expressed at higher levels. |
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Revision as of 21:21, 5 May 2011
Gene encoding IclR
IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. Therefore when pyruvate is present in high concentrations, the aceBAK promoter should be highly inhibited and downstream gene expression should be low. When pyruvate levels are low, the aceBAK promoter should be less inhibited and the downstream genes should be expressed at higher levels.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]