Difference between revisions of "Part:pSB1C3"
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__NOTOC__
 
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<partinfo>pSB1C3 short</partinfo>
 
<partinfo>pSB1C3 short</partinfo>
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High copy BioBrick assembly plasmid
 
  
 
pSB1C3 is a high copy number plasmid carrying chloramphenicol resistance.
 
pSB1C3 is a high copy number plasmid carrying chloramphenicol resistance.
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===Usage and Biology===
 
===Usage and Biology===
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In Spring 2011, pSB1C3 was sequenced using several primers. The current sequence reflects the most recent version (pSB1C3 w/ BBa_J04450, located in SP 4000 Well 2A).
  
 
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Revision as of 17:57, 4 May 2011

High copy BioBrick assembly plasmid

pSB1C3 is a high copy number plasmid carrying chloramphenicol resistance.

The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Usage and Biology

In Spring 2011, pSB1C3 was sequenced using several primers. The current sequence reflects the most recent version (pSB1C3 w/ BBa_J04450, located in SP 4000 Well 2A).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2049
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2049
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.