Difference between revisions of "Part:BBa K399001:Design"
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===Source=== | ===Source=== | ||
| − | mStrawberry was developed by Roger Tsien<sup>1</sup> | + | mStrawberry was developed by Roger Tsien.<sup>1</sup> |
===References=== | ===References=== | ||
1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572 | 1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572 | ||
Revision as of 01:51, 12 March 2011
mStrawberry
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 352
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 352
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 352
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.
Emission Spectrum
Characteristic emission spectrum for mStrawberry, showing its percent increase in fluorescence over that of DH5 alhpa cells at specific wavelengths. As expected, peak emission occurs at 600nm.1
Source
mStrawberry was developed by Roger Tsien.1
References
1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572