Difference between revisions of "Part:BBa K305003"

Revision as of 21:37, 7 November 2010

Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. It contains a hydrophobic chaplin domain and a signaling sequence.

Usage and Biology

The chaplins originate from Streptomyces coelicolor, which uses a variety of chaplins for the enforcement of its aerial hyphae.

There have to be found a variety of chaplains which can roughly be divided in two subcategories. The first is the group ranging from chaplin A till C. These are around 225 amino acids long and contain contain two hydrophobic chaplin domains, a hydrophilic region and a cell wall anchor as well as a signaling sequence. The second group of chaplins consist of chaplin D till H, which are approximately 63 amino acids long. These contain only one hydrophobic chaplin domain and a signaling sequence.

Chaplins can assemble into polymer chains, which form rod-like structures called amyloid fibers. These fibers are very rigid and hard to break down and can only be broken when boiled in SDS or with TCA and TFA treatment. They share distinguishing features with the medically important pathogenic amyloid fibers that are characteristic for many neurodegenerative diseases such as Alzheimer's, Huntington's, systemic amyloidosis and the prion diseases.

Physical properties

Interestingly, purified chaplins can be used to hydrophillically coat normally hydrophobic surfaces such as petri dishes. This is due to their amphipatic nature. This amphipatic property also gives them oil dispersing abilities.

Reference: Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726

Part Characterization

This graph shows the emission of Thioflavin T (ThT) stained samples of non-induced and induced B. subtilis strains with the E chaplin in the spaRK backbone (BBa_K305011). Induction was performed at an OD of 0.5 with 1,5% subtilin containing supernatant. After 6 hours after induction cell cultures were harvested and pellets were disrupted and boiled in SDS to remove other proteins. These samples were then freeze dried and the chaplins were monomerized using 99% TFA acid. To reassemble the monomerized chaplins into amyloid fibrils the chaplins were then diluted in demi water and vortexed for 1 min. These samples were then stained with Thioflavin T to a concentration of 3 microMolair.

Fluoresce emission was measured between 460-600 nm at an excitation freq of 450 nm. The induced strain (in the graph indicated as DE1) shows a clear emission peak around 482 nm as you expect when ThT binds to amyloid fibrils formed by the chaplins. In the non-iduced(in the graph indicated as "DEN") shows no such emissions at the same wavelength. The blank consists of H₂O with the addition of ThT at same concentration as in the other samples.

Sequence and Features