Difference between revisions of "Part:BBa K313007"

Latest revision as of 20:55, 7 November 2010

gfp generator in the reverse direction

This part is a gfp generator in reverse direction. It contains terminator. It is basically the reversed version of BBa_E0840.

verificatino experiment

The part is ligated with inverted pBAD promoter(BBa_J44002) and the plasmid was transformed into JM109.

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2. The result is shown below with the data of negative control that contains pBAD(reverse) and terminator.

The result of gfp expression assay

It is confirmed from the graph that this part properly worked.

Please see our presenteation slide and poster for our experiments utilized this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 209

@@ Line 7: / Line 7: @@
 === verificatino experiment ===
-The part is ligated with inverted pBAD promoter(BBa_K???) and the plasmid was transformed into JM109.
+The part is ligated with inverted pBAD promoter(BBa_J44002) and the plasmid was transformed into JM109.
+Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2. The result is shown below with the data of negative control that contains pBAD(reverse) and terminator.
 <gallery>
 Image:Gfp.png|The result of gfp expression assay
@@ Line 15: / Line 16: @@
 </gallery>
-Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.
-It is confirmed
+It is confirmed from the graph that this part properly worked.
 Please see our presenteation slide and poster for our experiments utilized this part.