Difference between revisions of "Part:BBa K342001:Design"
(→Design Notes) |
(→Source) |
||
| Line 13: | Line 13: | ||
<html> | <html> | ||
Synthesized (M. Gene)<br> | Synthesized (M. Gene)<br> | ||
| − | phaC from <i>Ralstonia eutropha H16</i><br> | + | <i>phaC</i> from <i>Ralstonia eutropha H16</i><br> |
</html> | </html> | ||
===References=== | ===References=== | ||
“Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 Poly-beta-hydroxybutyrate biosynthetic pathway”, Steven C. Slater, William H. Voige and Douglas E. Dennis, Oct. 1988, Journal Of Bacteriology, p. 4431–4436. | “Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 Poly-beta-hydroxybutyrate biosynthetic pathway”, Steven C. Slater, William H. Voige and Douglas E. Dennis, Oct. 1988, Journal Of Bacteriology, p. 4431–4436. | ||
Revision as of 17:55, 7 November 2010
Pha C (poly-β-hydroxybutyrate polymerase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 594
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 320
Illegal NgoMIV site found at 554
Illegal NgoMIV site found at 866
Illegal NgoMIV site found at 1145
Illegal NgoMIV site found at 1558
Illegal NgoMIV site found at 1625
Illegal AgeI site found at 293 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence comes from the first chromosome of bacteria Ralstonia eutropha H16 (old named Alcaligenes eutrophus), (reference: NC_008313), adapted to become a standard BioBrick (that is to say, without forbidden restriction sites).
Source
Synthesized (M. Gene)
phaC from Ralstonia eutropha H16
References
“Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 Poly-beta-hydroxybutyrate biosynthetic pathway”, Steven C. Slater, William H. Voige and Douglas E. Dennis, Oct. 1988, Journal Of Bacteriology, p. 4431–4436.