Difference between revisions of "Part:BBa K361001"
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In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
 
In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
  
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[[Image:Our message structure.png]]
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[[Image:Our_message_structure.png|thumb|"Fig.1 - The structure of our message used in the validation process"]]
  
  

Revision as of 03:26, 7 November 2010

Message gene template

This message system consists of a message sequence, with specific repeat sequences (repeat A: repeat 1 and 2) inserted as to be recognized by the Rci site-specific recombinase.

  • This message has 70 characters - "We must learn to live together as brothers or perish together as tools"
  • In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

"Fig.1 - The structure of our message used in the validation process"


  • Reference:

[1] Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
    Illegal BamHI site found at 427
    Illegal XhoI site found at 433
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]