Difference between revisions of "Part:BBa K353011"
(→‎Usage and Biology)
Line 11: Line 11:
 
<partinfo>BBa_K353011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K353011 SequenceAndFeatures</partinfo>
 
TGTG GTTTACCATT GTGCGTGTGG TTGGAGGTGT TGTGTTTGGT ATTTGGGTTT GGAGGCTGTG TTACTAGAGA AAGAGGAGAA A
 
TGTG GTTTACCATT GTGCGTGTGG TTGGAGGTGT TGTGTTTGGT ATTTGGGTTT GGAGGCTGTG TTACTAGAGA AAGAGGAGAA A
 +
  
  

Revision as of 21:46, 6 November 2010

sRNA1

This part is complementary to RSID 1 (BBa_....) and RBS BBa_B0034. The transcribed sRNA is designed to prevent the translation of an mRNA transcript containing RSID 1 and RBS_B0034 by binding to the mRNA transcript and occluding the RBS. This part does not have significant sequence similarity to the E. coli genome, so it should not interfere with the host cell machinery. For increased transcript stability, this part contains custom designed 5' hairpin to prevent RNase degradation.

Usage and Biology

Designed to be used in conjunction with RSID 1(BBa...) and RBS BBa_B0034. When used with promoters other than BBa_F2620, this part may be less stable because the 5' hairpin may not form correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

TGTG GTTTACCATT GTGCGTGTGG TTGGAGGTGT TGTGTTTGGT ATTTGGGTTT GGAGGCTGTG TTACTAGAGA AAGAGGAGAA A