Difference between revisions of "Part:BBa K500002:Experience"
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Spectrophotometry | Spectrophotometry | ||
| − | In order to measure the enzyme activity of Lac, we use ABTS as the substrate.The assay mixer contained 1mM ABTS and 0.1M sodium citrate buffer, pH3.0. Oxidation of ABTS WAS followed by absorbance increase at 420nm. | + | In order to measure the enzyme activity of Lac, we use ABTS as the substrate. |
| + | The assay mixer contained 1mM ABTS and 0.1M sodium citrate buffer, pH3.0. | ||
| + | Oxidation of ABTS WAS followed by absorbance increase at 420nm. | ||
Revision as of 21:31, 6 November 2010
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Applications of BBa_K500002
Headline text
Spectrophotometry
In order to measure the enzyme activity of Lac, we use ABTS as the substrate.
The assay mixer contained 1mM ABTS and 0.1M sodium citrate buffer, pH3.0. Oxidation of ABTS WAS followed by absorbance increase at 420nm.