Difference between revisions of "Part:BBa K313017"

Revision as of 19:32, 5 November 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

verification experiment

This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313008 is able to catalyze site specific recombination reaction.

Please see our [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 478
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 164
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
-Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
@@ Line 14: / Line 12: @@
 This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313008 is able to catalyze site specific recombination reaction.
+Please see our [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page for detail.
 <!-- Add more about the biology of this part here