Difference between revisions of "Part:BBa K371053"
 
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<partinfo>BBa_K371053 short</partinfo>
 
<partinfo>BBa_K371053 short</partinfo>
  
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This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. This part is used to make a plasmid compatible to RFC 53 in addition to RFC 10. So plasmid with this part complies with both 10 and 53 assembly standard. Then protein fusion can easily be done in this new plasmid.
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This part locate in plasmid backbone BBa_K371051, which is very like pSB1C3 with only a single base pair  mutation between terminator 0 and chloramphenicol resistance gene. This part is used to make a plasmid compatible to RFC 53 in addition to RFC 10. So plasmid with this part complies with both 10 and 53 assembly standard. Then protein fusion can easily be done in this new plasmid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:33, 1 November 2010

theRFC 10 and RFC53 interface converter

This part locate in plasmid backbone BBa_K371051, which is very like pSB1C3 with only a single base pair mutation between terminator 0 and chloramphenicol resistance gene. This part is used to make a plasmid compatible to RFC 53 in addition to RFC 10. So plasmid with this part complies with both 10 and 53 assembly standard. Then protein fusion can easily be done in this new plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 14
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3