Difference between revisions of "Part:BBa K339009"
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<partinfo>BBa_K339009 short</partinfo> | <partinfo>BBa_K339009 short</partinfo> | ||
− | The CpxR promoter | + | The CpxR promoter driving expression of red fluorescent protein (RFP). Downstream of this is an arabinose inducible promoter driving the expression of mutant maltose binding protein (malE31) with a storng ribosomal binding site. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | malE31 is a mutant form of the maltose binding protein, a native protein to E''scherichia Coli''. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K339009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K339009 SequenceAndFeatures</partinfo> |
Latest revision as of 00:49, 31 October 2010
CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31
The CpxR promoter driving expression of red fluorescent protein (RFP). Downstream of this is an arabinose inducible promoter driving the expression of mutant maltose binding protein (malE31) with a storng ribosomal binding site.
Usage and Biology
malE31 is a mutant form of the maltose binding protein, a native protein to Escherichia Coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2137
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2603
Illegal BamHI site found at 2076
Illegal BamHI site found at 2339 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 636
Illegal AgeI site found at 748
Illegal AgeI site found at 1911 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2301
Illegal SapI site found at 1893