Difference between revisions of "Part:BBa K371052"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K371052 short</partinfo>
 
<partinfo>BBa_K371052 short</partinfo>
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The function of this biobrick is to convert the any RFC53 part to RFC 10 part without leaving any trace. Besides it can also directly express the coding sequence by induced by IPTG. User will find it is very convenient to make fusion protein with this noval system.
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This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. The function of this gene is to convert the any RFC53 part to RFC 10 part without leaving any trace. User will find it is very convenient to make fusion protein with this noval system.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:52, 28 October 2010

RFC 53 easy expression biobrick The function of this biobrick is to convert the any RFC53 part to RFC 10 part without leaving any trace. Besides it can also directly express the coding sequence by induced by IPTG. User will find it is very convenient to make fusion protein with this noval system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 238
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 244
    Illegal SapI.rc site found at 231