Difference between revisions of "Part:BBa K200029:Experience"
 
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===Experience of UW-Madison 2010===
 
===Experience of UW-Madison 2010===
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The following is a zip file containing pertinent testing protocols and results, sequencing, and genbank files:  [[media:BBa_K318502_pSB1C3.zip]]
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The following is a zip file containing pertinent testing protocols and results, sequencing, and genbank files:  [[media:BBa_K318501_pSB1C3.zip]]
 
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[[image:UW_L_Fucose.jpg|400px]]
 
[[image:UW_L_Fucose.jpg|400px]]
  
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'''Figure 1:''' L-Fucose content of plasmids K318500, K318501(K200029), and K200021 (control) in backbone pSB1AK3 transformed in MG1655
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'''Figure 1:''' L-Fucose content of plasmids K318500, K318501(K200029), and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content.
 
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[[image:UW_cell_survivability.jpg|400px]]
 
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'''Figure 1:''' Survivability of plasmids K318500, K318501(K200029), K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We found different results compared to the survivability testing preformed by Imperial 2009. We saw around a 10x increase in survivability with RcsB being over produced at a ph=4.  
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'''Figure 2:''' Survivability of plasmids K318500, K318501(K200029), K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We found different results compared to the survivability testing preformed by Imperial 2009. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4.  
 
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For more detail on testing, please see our results page on our <html><a href="http://2010.igem.org/Team:Wisconsin-Madison">Team Wiki</a></html> for protocols, background information, and conclusions.
 
For more detail on testing, please see our results page on our <html><a href="http://2010.igem.org/Team:Wisconsin-Madison">Team Wiki</a></html> for protocols, background information, and conclusions.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 21:40, 28 October 2010

Experience of UW-Madison 2010

The following is a zip file containing pertinent testing protocols and results, sequencing, and genbank files: media:BBa_K318501_pSB1C3.zip

UW L Fucose.jpg

Figure 1: L-Fucose content of plasmids K318500, K318501(K200029), and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content.
UW cell survivability.jpg
Figure 2: Survivability of plasmids K318500, K318501(K200029), K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We found different results compared to the survivability testing preformed by Imperial 2009. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4.


For more detail on testing, please see our results page on our Team Wiki for protocols, background information, and conclusions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Applications of BBa_K200029

User Reviews

UNIQb9ea3238a8018e50-partinfo-00000002-QINU UNIQb9ea3238a8018e50-partinfo-00000003-QINU