Difference between revisions of "Part:BBa K322237:Experience"

(Applications of BBa_K322237)
(Applications of BBa_K322237)
 
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===Applications of BBa_K322237===
 
===Applications of BBa_K322237===
 
[[Image:WTlucie-ph7.jpg]]
 
[[Image:WTlucie-ph7.jpg]]
 +
 
This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in.
 
This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in.
  
 
[[Image:WTlucie-ph4.8.jpg]]
 
[[Image:WTlucie-ph4.8.jpg]]
 +
 
This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase.
 
This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase.
  

Latest revision as of 00:56, 28 October 2010

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Applications of BBa_K322237

WTlucie-ph7.jpg

This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in.

WTlucie-ph4.8.jpg

This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase.

User Reviews

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