Difference between revisions of "Part:BBa C0600:Design"
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===Design Notes===
 
===Design Notes===
  
−
 
+
This part had XbaI site. The site is cleared by introducing a silent mutation.
−
 
+
TCTAGA(at 420) has been changed to CCTAGA.
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A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome.
  
 
===Source===
 
===Source===

Latest revision as of 07:00, 5 September 2006


Tar


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

This part had XbaI site. The site is cleared by introducing a silent mutation. TCTAGA(at 420) has been changed to CCTAGA. A codon TAT(Tyr) is changed to TAC(Tyr).Both codons have around 50% abundance in E coli genome.

Source

References