Difference between revisions of "Part:BBa K395705"
 
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[[Image:Tokyotech registry-asta.jpg|left|700px|thumb|Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC<br>This work is done by Yumiko Kinoshita]]
 
[[Image:Tokyotech registry-asta.jpg|left|700px|thumb|Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC<br>This work is done by Yumiko Kinoshita]]
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Latest revision as of 00:11, 28 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.

Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC
This work is done by Yumiko Kinoshita





















In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715
    Illegal SapI site found at 542
    Illegal SapI site found at 554