Difference between revisions of "Part:BBa K404319"
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| − | ! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404319 | + | ! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404319 Cyan fluorescent protein] |
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] | |[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] | ||
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Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. | Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. | ||
| − | mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has | + | mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has higher brightness level, acid insensivity and greater photostability. |
Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles. | Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles. | ||
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Latest revision as of 01:26, 28 October 2010
Cyan fluorescent protein (CFP) in RFC25 standard
| Cyan fluorescent protein | |
|---|---|
| BioBrick Nr. | BBa_K404319 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | |
| Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has higher brightness level, acid insensivity and greater photostability. Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.
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Fig.1: Emission spectra and absorbance CFP
http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg
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Fig.2:Chromophor structure of CFP |
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Fig.3: Fluorescent protein beta-barrel architecture and approximate dimensions |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]