Difference between revisions of "Part:BBa K353002:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Karina Padilla, Francisco Cai, Laura O'Brien and Christina Smolke designed the RSID1 tag. It was developed as to not interfere with E. coli's native genome and to be bound by sRNA1 (see part K353009). It also contains a ribosome binding site (B0034).
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Karina Padilla, Francisco Cai, Laura O'Brien and Christina Smolke designed the RSID1 tag. It was developed to be bound by sRNA1 (see part K353009) and to be orthogonal to the E. coli genome. It also contains a ribosome binding site (B0034).
  
 
===Source===
 
===Source===

Latest revision as of 22:02, 6 November 2010

I0500 + K353010 + B0034 + E0040 + B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1953
    Illegal SapI site found at 961


Design Notes

Karina Padilla, Francisco Cai, Laura O'Brien and Christina Smolke designed the RSID1 tag. It was developed to be bound by sRNA1 (see part K353009) and to be orthogonal to the E. coli genome. It also contains a ribosome binding site (B0034).

Source

This part is assembled from other parts found in the registry (except for the RSID tag).

References