Difference between revisions of "Part:BBa K353002"

Latest revision as of 22:45, 27 October 2010

I0500 + K353010 + B0034 + E0040 + B1006

This part is composed of an inducible arabinose promoter (I0500), an RNA tag (K353010), GFP (E0040), and a terminator (B1006). This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of an mRNA transcript, and if no sRNA1 is present (see part K353011), this can then be translated into GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1953
Illegal SapI site found at 961

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K353002 short</partinfo>
-This part is composed of an inducible arabinose promoter (I0500), an RNA tag (K353010), a ribosome binding site (B0034), GFP (E0040), and a terminator (B1006). This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of an mRNA transcript, and if no sRNA1 is present (see part K353011), this can then be translated into GFP.
+This part is composed of an inducible arabinose promoter (I0500), an RNA tag (K353010), GFP (E0040), and a terminator (B1006). This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of an mRNA transcript, and if no sRNA1 is present (see part K353011), this can then be translated into GFP.
 <!-- Add more about the biology of this part here